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Specimen Requirements

Flow Cytometry

  • Peripheral blood- 3 ml sodium heparin - preferred (green top) or 3 ml in EDTA (purple top)
  • Bone Marrow Aspirate: 1-2 ml sodium heparin - preferred (green top) or 1-2 ml in EDTA (purple top)
  • Tissue: Multiple 3-4 mm pieces of tissue in transport media
  • Body Fluids: Mix 1:1 in RPMI transport media
Cytogenetics
  • Peripheral Blood: 5 ml in sodium heparin (green top)
  • Bone Marrow Aspirate: 1-2 ml in sodium heparin (green top)
  • Cord Blood: 2-5 ml in sodium heparin (green top)
  • Tissue: 5-10 mm or larger sized tissue in transport media
FISH
  • Peripheral Blood: 3 ml sodium heparin - preferred (green top) or 3 ml in EDTA (purple top)
  • Bone Marrow Aspirate: 1-2 ml sodium heparin - preferred (green top) or 3 ml in EDTA (purple top)
  • Tissue: Please call for additional specimen requirements
Molecular Analysis (PCR)
  • Peripheral Blood: 5 ml EDTA (purple top) preferred; ACD (yellow top) acceptable
  • Tissue: Minimum of 250 mg tissue in transport media
  • Formalin fixed paraffin embedded tissue
Molecular Analysis (PCR)
  • Formalin fixed paraffin embedded tissue
  • Tissue in 10% neutral buffered formalin


CSI Cytogenetics

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